Anti-Bid Cleavage Site抗体
兔多克隆抗体to Bid Cleavage Site
Rabbit
适用于: ICC, WBmore details
与反应: Mouse, Human
Synthetic peptide corresponding to Mouse Bid Cleavage Site (N terminal). Synthetic peptide (Mouse)corresponding to N-terminus of cleavage site (59/60).
Database link: P70444
WB: 3T3-L1 cells extracts. ICC: A549 cells.
BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl 2 family. BID interacts with both Bcl 2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Immunogen affinity purified
Purified from rabbit serum by epitope-specific affinity chromatography.
BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl 2 family. BID interacts with both Bcl 2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
多克隆
IgG
Abpromise™承诺保证使用ab10640于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | 1/250. | |
| WB | (1) | 1/1000. Predicted molecular weight: 15 kDa. |
Entrez Gene: 637 Human
Entrez Gene: 12122 Mouse
Omim: 601997 Human
SwissProt: P55957 Human
SwissProt: P70444 Mouse
BID antibody
BID isoform ES(1b) antibody
BID isoform L(2) antibody
BID isoform Si6 antibody
Desmocollin type 4 antibody
FP497 antibody
Human BID coding sequence antibody
MGC15319 antibody
MGC42355 antibody
p22 BID antibody
Apoptic death agonist antibody
BH 3 interacting domain death agonist antibody
BH3 interacting domain death agonist antibody
Western blot - Anti-Bid Cleavage Site antibody (ab10640)
All lanes : Anti-Bid Cleavage Site antibody (ab10640) at 1/1000 dilution
Lane 1 : 3T3-L1 cells without BID
Lane 2 : 3T3-L1 cells with caspase-8 cleaved recombinant mouse BID
Lane 3 : 3T3-L1 cells with caspase-8 cleaved recombinant human BID
Predicted band size: 15 kDa
SDS-PAGE on a 4-20% Tris-glycine gel
Immunocytochemistry - Anti-Bid Cleavage Site antibody (ab10640)
A549 cells stained for BID cleave site (green) using ab10640 at 1/250 dilution in ICC/IF. It was followed by Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody at 1/400 dilution for 30 minutes at room temperature (Panel a). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI. F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e shows no primary antibody control.
