Anti-CD45 antibody - Hematopoietic Stem Cell Marker

• 产品名称

  Anti-CD45抗体- Hematopoietic Stem Cell Marker
  参阅全部 CD45 一抗

• 描述

  兔多克隆抗体to CD45 - Hematopoietic Stem Cell Marker

• 宿主

  Rabbit

• 经测试应用

  适用于: WB, IHC-Pmore details

• 种属反应性

  与反应: Human
  预测可用于: Pig, Monkey

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 98.98% PBS, 1% BSA
  
  Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab10559于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5788 Human

• Omim: 151460 Human

• SwissProt: P08575 Human

• Unigene: 654514 Human

• 别名

• CD45 antigen antibody

• CD45R antibody

• GP180 antibody

• L-CA antibody

• LCA antibody

• Leukocyte common antigen antibody

• loc antibody

• Ly-5 antibody

• LY5 antibody

• Ly5, homolog of antibody

• Lyt-4 antibody

• OTTHUMP00000033813 antibody

• OTTHUMP00000033816 antibody

• OTTHUMP00000033817 antibody

• OTTHUMP00000038574 antibody

• Protein tyrosine phosphatase receptor type c polypeptide antibody

• Protein tyrosine phosphatase, receptor type C antibody

• protein tyrosine phosphatase, receptor type, C antibody

• Protein tyrosine phosphatase, receptor type, c polypeptide antibody

• Ptprc antibody

• PTPRC_HUMAN antibody

• Receptor-type tyrosine-protein phosphatase C antibody

• T200 antibody

• T200 glycoprotein antibody

• T200 leukocyte common antigen antibody

• B220 antibody

• CD 45 antibody

• CD45 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody - Hematopoietic Stem Cell Marker (ab10559)

  IHC image of ab10559 staining in human Hodgkins lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10559, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Western blot - Anti-CD45 antibody - Hematopoietic Stem Cell Marker (ab10559)

  ab10559  rabbit polyclonal to CD45  (1/500) with secondary goat anti-rabbit IgG ab6721 (1/5000).

  Expected molecular weight: 147 kDa. WB exposure time: 1min 30sec    

  Lanes 1 to 10: 20µg of cell lysate per lane
  Lanes 6 to 10: ab17550 CD45 blocking peptide used at 1µg/ml

  Lane 1: HeLa Nuclear Extract (ab10559)
  Lane 2: HeLa Whole Cell Lysate (ab10559)
  Lane 3: A431 Whole Cell Lysate (ab10559)
  Lane 4: Jurkat Whole Cell Lysate (ab10559)
  Lane 5: HEK293 Whole Cell Lysate (ab10559)
  Lane 6: HeLa Nuclear Extract (ab10559+ ab17550)
  Lane 7: HeLa Whole Cell Lysate (ab10559+ ab17550)
  Lane 8: A431 Whole Cell Lysate (ab10559+ ab17550)
  Lane 9: Jurkat Whole Cell Lysate (ab10559+ ab17550)
  Lane 10: HEK293 Whole Cell Lysate (ab10559+ ab17550)

  A strong band, slightly higher was seen in Jurkat cell lysate. The band was blocked using the immunising peptide (ab17550). It is likely that the band is CD45.