Anti-Nucleophosmin antibody [FC82291]

• 产品名称

  Anti-Nucleophosmin抗体[FC82291]
  参阅全部 Nucleophosmin 一抗

• 描述

  小鼠单克隆抗体[FC82291] to Nucleophosmin

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Dog, Human, African green monkey, Chinese hamster
  

• 免疫原

  Full length native protein (purified) corresponding to Rat Nucleophosmin.

• 表位

  The epitope recognized by the antibody lies within the 68 amino acids at the C terminus of B23.

• 阳性对照

• NIH3T3 whole cell lysate.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituents: 0.0268% PBS, 1% BSA

• 浓度

• 批次浓度范围 50 µl 浓度为 0.5 - 0.6 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  FC82291

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab10530于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 614028 Cow

• Entrez Gene: 4869 Human

• Entrez Gene: 18148 Mouse

• Entrez Gene: 25498 Rat

• Omim: 164040 Human

• SwissProt: P06748 Human

• SwissProt: Q61937 Mouse

• SwissProt: P13084 Rat

• Unigene: 557550 Human

• Unigene: 485384 Mouse

• Unigene: 54537 Rat

别名

• B23 antibody

• MGC104254 antibody

• NO38 antibody

• NPM antibody

• NPM_HUMAN antibody

• NPM1 antibody

• Nucleolar phosphoprotein B23 antibody

• Nucleolar protein NO38 antibody

• Nucleophosmin (nucleolar phosphoprotein B23 numatrin) antibody

• Nucleophosmin antibody

• nucleophosmin nucleoplasmin family member 1 antibody

• Nucleophosmin/nucleoplasmin family member 1 antibody

• Numatrin antibody

• OTTHUMP00000161024 antibody

• OTTHUMP00000161025 antibody

• OTTHUMP00000223397 antibody

• OTTHUMP00000223398 antibody

• 

  Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530)

  All lanes : Anti-BRD2 antibody [EPR7642] - ChIP Grade (ab139690) at 1/1000 dilution
  
  Lane 1 : Wild-type A549 ab288558 cytoplasm lysate
  Lane 2 : Wild-type A549 ab288558 nuclear lysate
  Lane 3 : BRD2 knockout A549 cytoplasm lysate
  Lane 4 : BRD2 knockout A549 nuclear lysate
  Lane 5 : Wild-type HEK-293T ab255553 cell lysate
  Lane 6 : BRD2 knockout HEK-293T cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Performed under reducing conditions.
  
  Predicted band size: 33 kDa
  
  
  
  

  False colour image of Western blot: Anti-BRD2 antibody [EPR7642] - ChIP Grade staining at 1/1000 dilution, shown in green; Mouse Anti-Nucleophosmin antibody [FC82291] (ab10530) loading control staining at 2 ug/mL imaged in ECL. In Western blot, ab139690 was shown to bind specifically to BRD2. A band was observed at 110 kDa in wild-type A549 cell lysates with no signal observed at this size in BRD2 knockout cell line. To generate this image, wild-type and BRD2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween®20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 20 seconds exposure time. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and HRP conjugated Goat anti-Mouse (H+L) at 1/20000 dilution.

• 

  Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530)This image is courtesy of an anonymous Abreview

  Western blot image of ab10530 staining whole cell lysate of U2OS cells.  The gel was blocked with 5% milk for 1 hour at 21°C. The primary antibody was diluted to 2 µg/ml and incubated  for 12 hours at 4°C.  A HRP conjugated goat anti-mouse antibody was used as the secondary.

  See Abreview

• 

  Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530)

  Anti-Nucleophosmin antibody [FC82291] (ab10530) at 0.2 µg/ml + 3T3 cell lysate
  
  Secondary
  Anti-Mouse IgG (Fab)
  
  Predicted band size: 33 kDa
  
  

• 

  Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530)

  All lanes : Anti-Nucleophosmin antibody [FC82291] (ab10530) at 0.5 µg/ml
  
  Lane 1 : HeLa cell lysate
  Lane 2 : JURKAT cell lysate
  Lane 3 : COS7 cell lysate
  Lane 4 : NIH-3T3 cell lysate
  Lane 5 : RAT2 cell lysate
  Lane 6 : CHO cell lysate
  Lane 7 : MDBK cell lysate
  Lane 8 : MDCK cell lysate
  
  Secondary
  All lanes : Goat Anti-Mouse IgG-Peroxidase
  
  Predicted band size: 33 kDa
  
  
  
  

  Cell line lysate separated on SDS-PAGE, probed with ab10530 (0.5 μg/mL)

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [FC82291] (ab10530)

  Immunocytochemistry/ Immunofluorescence analysis of HeLa cells staining Nucleophosmin using ab10530 (dilution 1/500). Cells were fixed and permeabilized with methanol followed by methanol:acetone. Developed using Goat Anti-Mouse IgG, Cy3™ conjugate and counterstained with DAPI (blue) to stain nuclei.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [FC82291] (ab10530)This image is courtesy of an Anonymous Abreview.

  ab10530 staining Nucleophosmin in immortalized Human trabecular meshwork cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 3% BSA for 45 minutes at room temperature. The primary antibody was diluted 1/1000 in PBS/0.3% BSA and incubated with the sample for 2 hours. The secondary antibody was Cy3®-conjugated Donkey anti-Mouse polyclonal, diluted 1/1000.

  See Abreview