Anti-CBX1 / HP1 beta抗体
参阅全部 CBX1 / HP1 beta 一抗
兔多克隆抗体to CBX1 / HP1 beta
Rabbit
ab10478 recognises a band at 25 kDa, which is attributed to CBX1 / HP1 beta.
适用于: WB, ICC/IF, IHC-P, IPmore details
与反应: Mouse, Rat, Human, Marsupial
预测可用于: Chicken, Hamster, Drosophila melanogaster
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
WB: HEK293T, HAP1, MCF7, A431, HeLa and PC12 cell lysates. IP: HeLa cell lysate.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab10478于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (5) | 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa). |
| ICC/IF | (3) | Use at an assay dependent concentration. |
| IHC-P | (2) | Use at an assay dependent concentration. |
| IP | Use at an assay dependent concentration. |
Entrez Gene: 10951 Human
Entrez Gene: 12412 Mouse
Omim: 604511 Human
SwissProt: P83916 Human
SwissProt: P83917 Mouse
Unigene: 77254 Human
Unigene: 29055 Mouse
CBX1_HUMAN antibody
Chromobox 1 antibody
Chromobox homolog 1 (HP1 beta homolog Drosophila ) antibody
chromobox homolog 1 antibody
Chromobox protein homolog 1 antibody
Drosophila HP1 beta antibody
Heterochromatin protein 1 beta antibody
Heterochromatin protein 1 homolog beta antibody
Heterochromatin protein p25 antibody
heterochromatin protein p25 beta antibody
HP1 beta antibody
HP1 beta homolog antibody
HP1 beta homolog Drosophila antibody
HP1, Drosophila. homolog of, beta antibody
HP1beta antibody
HP1Hs beta antibody
HP1Hsbeta antibody
Human heterochromatin protein p25 mRNA complete cds antibody
M31 antibody
MOD 1 antibody
MOD1 antibody
Modifier 1 protein antibody
p25beta antibody
CBX 1 antibody
CBX antibody
Cbx1 antibody
Western blot - Anti-CBX1 / HP1 beta antibody (ab10478)
All lanes : Anti-CBX1 / HP1 beta antibody (ab10478) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : CBX1 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 26 kDawhy is the actual band size different from the predicted?
Lanes 1- 2: Merged signal (red and green). Green - ab10478 observed at 26 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.
ab10478 was shown to react with CBX1 / HP1 beta in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266356 (knockout cell lysate ab257383) was used. Wild-type HEK-293T and CBX1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab10478 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-CBX1 / HP1 beta antibody (ab10478)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: CBX1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: MCF7 whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab10478 observed at 26 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab10478 was shown to recognize CBX1 when CBX1 knockout samples were used, along with additional cross-reactive bands. Wild-type and CBX1 knockout samples were subjected to SDS-PAGE. Ab10478 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-CBX1 / HP1 beta antibody (ab10478)
All lanes : Anti-CBX1 / HP1 beta antibody (ab10478) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa nuclear lysate
Lane 3 : A431 cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : HeLa whole cell lysate with CBX1 / HP1 beta peptide (ab30861) at 1 µg/ml
Lane 7 : HeLa nuclear lysate with CBX1 / HP1 beta peptide (ab30861) at 1 µg/ml
Lane 8 : A431 lysate with CBX1 / HP1 beta peptide (ab30861) at 1 µg/ml
Lane 9 : MCF7 cell lysate with CBX1 / HP1 beta peptide (ab30861) at 1 µg/ml
Lane 10 : HEK293 cell lysate with CBX1 / HP1 beta peptide (ab30861) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Predicted band size: 21 kDa
Observed band size: 25 kDawhy is the actual band size different from the predicted?
Additional bands at: 37 kDa (possible cross reactivity), 47 kDa (possible cross reactivity)
Immunoprecipitation - Anti-CBX1 / HP1 beta antibody (ab10478)
CBX1 / HP1 beta was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to CBX1 / HP1 beta and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10478.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 20ka: CBX1 / HP1 beta.
Western blot - Anti-CBX1 / HP1 beta antibody (ab10478)
Anti-CBX1 / HP1 beta antibody (ab10478) at 1 µg/ml + PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 27 kDawhy is the actual band size different from the predicted?
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Western blot - Anti-CBX1 / HP1 beta antibody (ab10478)
Anti-CBX1 / HP1 beta antibody (ab10478) at 1/500 dilution + Recombinant Human CBX1 / HP1 beta protein (ab109847) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 10 seconds
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