Anti-SMC2抗体
参阅全部 SMC2 一抗
兔多克隆抗体to SMC2
Rabbit
适用于: WB, IPmore details
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide. The epitope maps to a region between residue 1150 and the C-terminus (residue 1197) of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592).
IP: HeLa cell lysates; WB: HeLa and HEK293 cell lysates
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab10412于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 137 kDa). | |
| IP | Use a concentration of 1 µg/ml. |
Entrez Gene: 10592 Human
Omim: 605576 Human
SwissProt: O95347 Human
Unigene: 119023 Human
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Western blot - Anti-SMC2 antibody (ab10412)
All lanes : Anti-SMC2 antibody (ab10412) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK293 cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 137 kDa
Exposure time: 3 seconds
Immunoprecipitation - Anti-SMC2 antibody (ab10412)
Immunoprecipitation of Human Aurora A with ab10412 at 1 µg/mL.
Lane 1: ab10412 + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lane 2: IgG control + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Detection: Chemiluminescence
