Anti-hnRNP H抗体
参阅全部 hnRNP H 一抗
兔多克隆抗体to hnRNP H
Rabbit
The antibody was designed against hnRNPH1 and does not recognize hnRNPH3. The epitope recognized by ab10374 maps to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneID 3187). This product may cross-react with hnRNPH2.
适用于: WB, IHC-P, IP, ICC/IF, IHC-Frmore details
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
Synthetic peptide corresponding to Human hnRNP H aa 400 to the C-terminus (C terminal).
Database link: 3187
IP: HeLa whole cell lysate WB: HeLa, HEK-293T, and NIH/3T3 cell lysates.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab10374于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) | 1/10000 - 1/30000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa). |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IP | (1) | Use at 4-10 µg/mg of lysate. |
| ICC/IF | (2) | Use at an assay dependent concentration. PubMed: 20020773 |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 24462217 |
Entrez Gene: 3187 Human
Entrez Gene: 59013 Mouse
Omim: 601035 Human
SwissProt: P31943 Human
SwissProt: O35737 Mouse
Unigene: 604001 Human
Unigene: 21740 Mouse
Unigene: 23677 Rat
DKFZp686A15170 antibody
Heterogeneous nuclear ribonucleoprotein H antibody
Heterogeneous nuclear ribonucleoprotein H1 (H) antibody
Heterogeneous nuclear ribonucleoprotein H1 antibody
HNRH1_HUMAN antibody
hnRNP H antibody
hnRNPH antibody
Hnrnph1 antibody
HNRPH 1 antibody
HNRPH antibody
HNRPH1 protein antibody
N-terminally processed antibody
Western blot - Anti-hnRNP H antibody (ab10374)
All lanes : Anti-hnRNP H antibody (ab10374) at 0.2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody (ab10374)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling hnRNP H with ab10374 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - Anti-hnRNP H antibody (ab10374)
Detection of human hnRNP H by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells.
Lane 1: Rabbit polyclonal to hnRNP H (ab10374) at 0.2 µg/mg lysate current batch
Lane 2: Rabbit polyclonal to hnRNP H (ab10374) at 0.2 µg/mg lysate former batch.
Lane 3: Control IgG
ab10374 was used at 1 ug/mL for Western blot.
Detection: Chemiluminescence with an exposure time of 30 seconds.
