Anti-SRC3抗体
参阅全部 SRC3 一抗
兔多克隆抗体to SRC3
Rabbit
适用于: IP, WBmore details
与反应: Human
预测可用于: Chimpanzee, Orangutan
Synthetic peptide within Human SRC3 aa 900-1000. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9Y6Q9
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. SRC3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. SRC3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.
多克隆
IgG
Abpromise™承诺保证使用ab10310于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use a concentration of 1 - 4 µg/ml. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 170 kDa). |
Entrez Gene: 8202 Human
Omim: 601937 Human
SwissProt: Q9Y6Q9 Human
Unigene: 592142 Human
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Immunoprecipitation - Anti-SRC3 antibody (ab10310)
Detection of SCR3 by Immunoprecipitation.
Samples: Nuclear extract (10 mg) from HeLa cells. Antibody used at 20 µg/ml extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC3.
Western blot - Anti-SRC3 antibody (ab10310)
Detection of SRC3 by Western Blot.
Samples: Nuclear extract (5 µg) from HeLa cells. Primary antibody ab10310 used at 0.33 µg/ml. Detection: Alkaline phosphatase conjugated goat anti-rabbit IgG diluted 1/3000 with NBT-BCIP as substrate for 20 min.
Detection of SCR3 by Immunoprecipitation. Samples: Nuclear extract (10 mg) from HeLa cells. Antibody used at 20 µg/ml extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC3.
