Anti-Transportin 1/MIP antibody [D45]

• 产品名称

  Anti-Transportin 1/MIP抗体[D45]
  参阅全部 Transportin 1/MIP 一抗

• 描述

  小鼠单克隆抗体[D45] to Transportin 1/MIP

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, ICC/IF, IHC-P, WBmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant fragment corresponding to Human Transportin 1/MIP.
  Database link: Q92973

• 阳性对照

• WB: HeLa and SW480 whole cell lysate. IHC-P: Human pancreas tissue. Flow Cyt: HeLa cells. ICC/IF: HeLa cells.

• 常规说明

  Heterogeneous nuclear ribonucleoprotein hnRNPA1 is an abundant nuclear protein that plays an important role in pre-mRNA processing and mRNA export from the nucleus. A1 shuttles rapidly between the nucleus and the cytoplasm, and a 38-amino acid domain, M9, serves as the bi-directional transport signal of A1. Recently, a 90-kD protein, transportin, was identified as the mediator of A1 nuclear import. Transportin mediates the nuclear import of additional hnRNP proteins, including hnRNPF. The nuclear localization of A1 is dependent on ongoing RNA polymerase II transcription.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  Preservative: 0.1% Sodium azide
  Constituent: PBS

• 纯度

  Protein A purified

• 纯化说明

  Protein A purified from tissue culture supernatant.

• Primary antibody说明

  Heterogeneous nuclear ribonucleoprotein hnRNPA1 is an abundant nuclear protein that plays an important role in pre-mRNA processing and mRNA export from the nucleus. A1 shuttles rapidly between the nucleus and the cytoplasm, and a 38-amino acid domain, M9, serves as the bi-directional transport signal of A1. Recently, a 90-kD protein, transportin, was identified as the mediator of A1 nuclear import. Transportin mediates the nuclear import of additional hnRNP proteins, including hnRNPF. The nuclear localization of A1 is dependent on ongoing RNA polymerase II transcription.

• 克隆

  单克隆

• 克隆编号

  D45

• 骨髓瘤

  Sp2/0

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab10303于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3842 Human

• Omim: 602901 Human

• SwissProt: Q92973 Human

• Unigene: 482497 Human

• 别名

• IPO 2 antibody

• IPO2 antibody

• Karyopherin (importin) beta 2 antibody

• Karyopherin beta 2 antibody

• Karyopherin beta-2 antibody

• KPN B2 antibody

• KPNB 2 antibody

• KPNB2 antibody

• M9 interacting protein antibody

• M9 region interacting protein antibody

• M9 region interaction protein antibody

• MIP 1 antibody

• MIP antibody

• MIP1 antibody

• OTTHUMP00000126960 antibody

• OTTHUMP00000222024 antibody

• OTTHUMP00000226224 antibody

• TNPO 1 antibody

• Tnpo1 antibody

• TNPO1_HUMAN antibody

• Transportin 1 antibody

• Transportin antibody

• Transportin-1 antibody

• Transportin1 antibody

• TRN antibody

• Importin 2 antibody

• Importin beta 2 antibody

• Importin beta-2 antibody

• 

  Western blot - Anti-Transportin 1/MIP antibody [D45] (ab10303)

  All lanes : Anti-Transportin 1/MIP antibody [D45] (ab10303) at 5 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Predicted band size: 107 kDa
  Observed band size: 107 kDa
  Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transportin 1/MIP antibody [D45] (ab10303)

  IHC image of ab10303 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10303, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  

• 

  Flow Cytometry - Anti-Transportin 1/MIP antibody [D45] (ab10303)

  Overlay histogram showing HeLa cells stained with ab10303 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10303, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Transportin 1/MIP antibody [D45] (ab10303)This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhury

  ab10303 staining cultured P0 mouse neurons by ICC/IF. The cultured neurons were fixed with 4% formaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% Triton X for 30 minutes  at 24°C. The cultured neurons were then stained with ab10303 at 1/1000 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 568 donkey anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody.  Hoechst was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Transportin 1/MIP antibody [D45] (ab10303)

  ICC/IF image of ab10303 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10303, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.