Anti-Podoplanin / gp36 antibody [18H5] - BSA and Azide free

• 产品名称

  Anti-Podoplanin抗体[18H5] - BSA and Azide free
  参阅全部 Podoplanin 一抗

• 描述

  小鼠单克隆抗体[18H5] to Podoplanin - BSA and Azide free

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, WB, Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length protein corresponding to Podoplanin. MDCK cells stably expressing Podoplanin protein.
  Database link: Q86YL7

• 阳性对照

• WB: human lymphatic endothelial cells, human placenta and skeletal muscle tissue lysates. ICC/IF: human dermal lymphatic endothelial Flow Cyt: HCT 116 (Human colorectal carcinoma cell line) whole cell lysate,

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Constituent: PBS

• 无载体

  是

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  18H5

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab10288于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 10630 Human

• Omim: 608863 Human

• SwissProt: Q86YL7 Human

• Unigene: 468675 Human

• 别名

• Glycoprotein 36 antibody

• gp 36 antibody

• GP 38 antibody

• GP 40 antibody

• gp36 antibody

• GP38 antibody

• GP40 antibody

• HT1A 1 antibody

• HT1A-1 antibody

• HT1A1 antibody

• hT1alpha 1 antibody

• hT1alpha 2 antibody

• hT1alpha1 antibody

• hT1alpha2 antibody

• Lung type I cell membrane associated glycoprotein antibody

• Lung type I cell membrane associated glycoprotein isoform a antibody

• Lung type I cell membrane associated glycoprotein T1A 2 antibody

• OTS 8 antibody

• OTS8 antibody

• OTTHUMP00000009640 antibody

• OTTHUMP00000044504 antibody

• PA2.26 antibody

• PA2.26 antigen antibody

• Pdpn antibody

• PDPN_HUMAN antibody

• Podoplanin antibody

• PSEC0003 antibody

• PSEC0025 antibody

• T1 alpha antibody

• T1 ALPHA GENE antibody

• T1-alpha antibody

• T1A 2 antibody

• T1A antibody

• T1A-2 antibody

• T1A2 antibody

• TI1A antibody

• TIA 2 antibody

• TIA2 antibody

• Aggrus antibody

• D2-40 antibody

• Glycoprotein 36 KD antibody

• 

  Immunocytochemistry/ Immunofluorescence - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)

  ab10288 at a 1/500 dilution staining human dermal lymphatic endothelial by Immunocytochemistry. The cells were grown in coverslips and fixed in formalin prior to permeabilization with tritonX-100. The cells were incubated with the primary antibody at 1/500 for 1 hour and then bound antibody was detected using a biotinylated goat anti-mouse antibody. Texas red-streptavidin was used for development.

  This image is courtesy of an Abreview submitted by Maria Navarro.

  See Abreview

• 

  Western blot - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)

  All lanes : Anti-Podoplanin antibody [18H5] - BSA and Azide free (ab10288) at 5 µg/ml
  
  Lane 1 : Human placenta tissue lysate - total protein (ab29745)
  Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 24 kDa
  Observed band size: 35 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 20 minutes
  
  
  Podoplanin protein is extensively glycosylated. This might explain the observation of a band at a higher molecular weight than predicted.

• 

  Flow Cytometry - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)

  Overlay histogram showing HCT116 cells stained with ab10288 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10288, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HCT116 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
  
  Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  

• 

  Flow Cytometry - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)

  Flow cytometric analysis of human primary lymphatic endothelial cells (HDLEC) labeling Podoplanin with ab10288 at 5 µg/mL. Anti-mouse IgG-PE was used as a secondary antibody.

   

   

• 

  Flow Cytometry - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)

  Flow cytometric analysis of primary human dermal microvascular (HDMVEC) and umbilical vein (HUVEC) endothelial cells labeling Podoplanin with ab10288 at 5 µg/mL. The lymphatic endothelial cell marker Podoplanin is not expressed in the blood endothelial cells.

• 

  Western blot - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)

  Western blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC) - lane 1, and HUVECs - lane 2. Total lysate of both cell types were subjected to SDS-PAGE and subsequent western blot analysis with ab10288. The antibody recognizes a protein of about 36 kDa in total lysate from LECs but not from HUVEC.

• 

  Immunocytochemistry/ Immunofluorescence - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)

  Immunocytochemistry/Immunofluorescence analysis of human dermal lymphatic endothelial cells (HDLEC) labelling Podoplanin with ab10288. An Alexa Fluor® 488-conjugated goat anti-mouse was used as the secondary antibody. Nuclei counterstained with DAPI.