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Human TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human TNF alpha in Human in Biofluids, Cell Culture Media samples.
隐藏别名
TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 30 pg/mL
24.58 - 6000 pg/mL
Microplate
Abcam's TNF alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TNF alpha in serum, plasma and cell culture supernatants. (Human TNF alpha concentration is pretty low in normal serum/plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human TNF alpha ELISA Kit (ab181421) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
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Representative standard curve using ab100654
Representative standard curve using ab100654
huTNFa measured in biological fluids, background signal subtracted (duplicates +/- SD).
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