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Human Soluble TNF Receptor I ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Soluble TNF Receptor I in Human in Biofluids, Cell Culture Media samples.
隐藏别名
CD120a, TNFAR, TNFR1, TNFRSF1A, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 1 pg/mL
0.55 - 400 pg/mL
Microplate
Abcam's Human STNF-R1 (soluble tumor necrosis factor receptor I) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human STNF-R1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human STNF-R1 coated on a 96-well plate. Standards and samples are pipetted into the wells and STNF-R1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human STNF-R1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of STNF-R1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
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-20°C
Representative standard curve using ab100642
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