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Human Sonic Hedgehog ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Sonic Hedgehog in Human in Biofluids, Cell Culture Media samples.
隐藏别名
Sonic hedgehog protein, SHH, HHG-1, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 20 pg/mL
13.72 - 10000 pg/mL
Microplate
Abcam's Sonic Hedgehog Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Sonic Hedgehog in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human Sonic Hedgehog coated on a 96-well plate. Standards and samples are pipetted into the wells and Sonic Hedgehog present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Sonic Hedgehog antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Sonic Hedgehog bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
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Representative standard curve using ab100639
Representative standard curve using ab100639
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