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Human P-Selectin (CD62P) ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human P-Selectin (CD62P) in Human in Biofluids, Cell Culture Media samples.
隐藏别名
CD62P, GMRP, GRMP, SELP, P-selectin, CD62 antigen-like family member P, Granule membrane protein 140, Leukocyte-endothelial cell adhesion molecule 3, Platelet activation dependent granule-external membrane protein, GMP-140, LECAM3, PADGEM
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 20 pg/mL
0.041 - 30 ng/mL
Microplate
Abcam's CD62P (P-Selectin) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD62P in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CD62P coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62P present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD62P antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62P bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Blue Ice
-20°C
-20°C
-20°C
Representative Standard Curve using ab100631.
Representative Standard Curve using ab100631.
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