| 存储条件 | -20℃ |
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Anti-Ki67 antibody [SP6] (ab16667) is a rabbit monoclonal antibody detecting Ki67 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF, mIHC. Suitable for Human, Mouse, Rat.
- Clone SP6 is the most cited clone to Ki67
- KO validated for confirmed specificity
- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency
隐藏别名
Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67
Rabbit
Monoclonal
SP6
IgG
No
Mouse, Rat, Human
IHC-P, ICC/IF, WB, mIHC, Flow Cyt (Intra)
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The exact immunogen used to generate this antibody is proprietary information.
C-terminus
Ki67 is mainly expressed in proliferating cells. For normal tissue samples (e.g., liver, kidney), no staining may be typically observed due to low level of proliferation and little expression of Ki67. For malignant tissue samples (e.g., colon carcinoma, breast carcinoma), it is more easily to find Ki67 in the proliferating cells of these tissues (PMID: 6206131, 10653597, 34183782).
FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the Product Protocol section.
0.033 mg/mL 本产品的浓度可能与批次相关
Liquid
Affinity purification Protein A
pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)](https://content.abcam.com/products/images/ki67-antibody-sp6-ab16667--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img417456.jpg/_jcr_content/renditions/webp-768.webp)
IHC image of ab16667 staining Ki67 in a section of formalin-fixed paraffin-embedded Human colon carcinoma tissue. The section incubated with ab16667 at 1/200 (0.145 μg/ml) dilution and ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) was used as secondary antobody. Positive staining on human colon carcinoma. The section was incubated with ab16667 at 4°C overnight. The section was counterstained with haematoxylin. Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)](https://content.abcam.com/products/images/ki67-antibody-sp6-ab16667--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433006.jpg/_jcr_content/renditions/webp-768.webp)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of formaldehyde fixed human pancreas staining with ab16667 at 1/100 dilution. Secondary antibody used was HRP BOND Refine Detection kit. Blocking was done with 5% serum for 1 hour at 20 °C. The sample was incubated with the primary antibody for 18 hours at 4°C with 1% BSA. Antigen retrieval method was heat mediated 10mm sodium citric buffer, PH 6
![Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [SP6] (AB16667)](https://content.abcam.com/products/images/ki67-antibody-sp6-ab16667--immunocytochemistry-immunofluorescence-img427392.jpg/_jcr_content/renditions/webp-768.webp)
ab16667 staining of Ki67 in a HCT116 cell spheroid. The cells were fixed with 100% methanol (5 min) permeabilised with 0.5% Triton X-100 for 1h and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween overnight at room temperature. The spheroids were then incubated overnight at room temperature with ab16667 at 2 μg/ml and ab7291 Mouse monoclonal [DM1A] to alpha Tubulin at 2 μg/ml. DAPI was used as nuclear counterstain (shown in blue). As secondary antibodies ab150081 Goat anti-Rabbit (Alexa Fluor® 488) (shown in green) and ab150120 Goat anti-Mouse (Alexa Fluor® 594) (shown in magenta) were used incubated overnight at room temperature. All permeabilization blocking and antibody incubation steps were performed using a rotary shaker.
Image was acquired with a high-content analyser (Operetta CLS Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
The antibody ab16667 also worked using 4% formaldehyde fixation (10 min).
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