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Human MIP1 beta ELISA Kit (CCL4) is a Sandwich (quantitative) ELISA for the measurement of Human MIP1 beta (CCL4) in Human in Biofluids, Cell Culture Media samples.
隐藏别名
LAG1, MIP1B, SCYA4, CCL4, C-C motif chemokine 4, G-26 T-lymphocyte-secreted protein, HC21, Lymphocyte activation gene 1 protein, MIP-1-beta(1-69), Macrophage inflammatory protein 1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2, LAG-1, MIP-1-beta, ACT-2
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 2.5 pg/mL
4.1 - 1000 pg/mL
Microplate
Abcam's MIP1 beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP1 beta in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human MIP1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman MIP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
This product may also recognise CC4L [C-C motif chemokine 4-like], a family member which shares high homology with CCL4.
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Representative Standard Curve using ab100597.
Representative Standard Curve using ab100597.
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