| 存储条件 | -20℃ |
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Human M-CSF ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human M-CSF in Human in Biofluids, Cell Culture Media samples.
隐藏别名
Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, CSF1
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 5 pg/mL
2.78 - 2000 pg/mL
Microplate
Abcam's MCSF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MCSF in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human MCSF coated on a 96-well plate. Standards and samples are pipetted into the wells and MCSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman MCSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Blue Ice
-20°C
-20°C
-20°C
Representative Standard Curve using ab100590.
Representative Standard Curve using ab100590.
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