Human IL-18BP ELISA Kit

Abcam's IL-18BP (Interleukin-18 Binding Protein) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18BP in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human IL-18BP coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-18BP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-18 BP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18BP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.