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Human DKK4 ELISA Kit (Dickkopf-4) is a Sandwich (quantitative) ELISA for the measurement of Human DKK4 (Dickkopf-4) in Human in Biofluids, Cell Culture Media samples.
隐藏别名
Dickkopf-related protein 4, Dickkopf-4, Dkk-4, hDkk-4, DKK4
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 10 pg/mL
13.72 - 10000 pg/mL
Microplate
Abcam's DKK4 (Dickkopf-4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK4 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human DKK4 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
Representative Standard Curve using ab100503.
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