| 存储条件 |
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| Product Name | CD53 [7A8D3] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | AI323659 antibody Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody CD 53 antibody CD53 antibody CD53 antigen antibody CD53 antigen, isoform CRA_b antibody CD53 glycoprotein antibody Cd53 molecule antibody CD53 tetraspan antigen antibody CD53_HUMAN antibody Cell surface glycoprotein CD53 antibody Leukocyte antigen MRC OX-44 antibody Leukocyte surface antigen CD53 antibody MOX 44 antibody MOX44 antibody OTTHUMP00000059505 antibody Ox-44 antibody OX44 antibody RATOX44 antibody Tetraspanin 25 antibody Tetraspanin-25 antibody Tetraspanin25 antibody Transmembrane glycoprotein antibody TSPAN 25 antibody Tspan-25 antibody TSPAN25 antibody |
| Product description | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants. |
| Immunogen | Purified recombinant fragment of human CD53 (AA: extra mix) expressed in E. Coli. |
| Clonality | Monoclonal |
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| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | AI323659 antibody Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody CD 53 antibody CD53 antibody CD53 antigen antibody CD53 antigen isoform CRA_b antibody CD53 glycoprotein antibody Cd53 molecule antibody CD53 tetraspan antigen antibody CD53_HUMAN antibody Cell surface glycoprotein CD53 antibody Leukocyte antigen MRC OX-44 antibody Leukocyte surface antigen CD53 antibody MOX 44 antibody MOX44 antibody OTTHUMP00000059505 antibody Ox-44 antibody OX44 antibody RATOX44 antibody Tetraspanin 25 antibody Tetraspanin-25 antibody Tetraspanin25 antibody Transmembrane glycoprotein antibody TSPAN 25 antibody Tspan-25 antibody TSPAN25 antibody |
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| Molecular Weight(MW) | 24.3kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P19397 |
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Application
Western blot analysis of CD53 against human CD53 (AA: extra mix) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-35, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-35 against HEK293 (1) and CD53 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-35, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-35 against Raji (1), Ramos (2), Jurkat (3), MOLT4 (4), K562 (5), HL-60 (6), THP-1 (7), and U937 (8) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-35, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-CD53 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1711-35, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CD53 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1711-35, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of CD53 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-35, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Raji, Ramos, Jurkat, MOLT4, K562, HL-60, THP-1, and U937 cell L-60 cells cervical cancer bladder cancer |
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| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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