| 存储条件 |
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| Product Name | Caspase-10 [JJ0890] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALPS2 antibody Apoptotic protease Mch-4 antibody CASP 10 antibody CASP-10 antibody CASP10 antibody CASPA_HUMAN antibody Caspase 10 apoptosis related cysteine peptidase antibody Caspase-10 subunit p12 antibody FADD like ICE2 antibody Fas associated death domain protein antibody FAS-associated death domain protein interleukin-1B-converting enzyme 2 antibody FLICE 2 antibody FLICE2 antibody ICE like apoptotic protease 4 antibody ICE-like apoptotic protease 4 antibody Interleukin 1B converting enzyme 2 antibody MCH 4 antibody |
| Product description | Caspase-10, also designated Mch4, is recruited to the native TRAIL and CD9 death-inducing signaling complexes (DISCs) by the FADD/Mort1 adaptor protein complex. Caspase-10 requires the assembly of the FADD and DISC complexes for its recruitment and cleavage-induced activation during CD95-induced apoptosis of activated T cells. The N-terminus of caspase-10 contains FADD-like death effector domains further indicating that it associates with FADD to induce apoptosis. Caspase-10 is not required for apoptosis induction and when overexpressed, cannot reverse defects in apoptosis induction caused by caspase-8 deficiency. Granzyme B cleaves procaspase-10 at an IXXD-A processing sequence to produce mature caspase-10. Mutations in the caspase-10 gene in the prodomain, p17 large protease subunit and p12 small protease subunit have been linked to a number of non-Hodgkin lymphomas in humans. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIPFC |
| WB:1:1,000-1:2,000 FC:1:50-1:100 IHC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ALPS2 antibody Apoptotic protease Mch-4 antibody CASP 10 antibody CASP-10 antibody CASP10 antibody CASPA_HUMAN antibody Caspase 10 apoptosis related cysteine peptidase antibody Caspase-10 subunit p12 antibody FADD like ICE2 antibody Fas associated death domain protein antibody FAS-associated death domain protein interleukin-1B-converting enzyme 2 antibody FLICE 2 antibody FLICE2 antibody ICE like apoptotic protease 4 antibody ICE-like apoptotic protease 4 antibody Interleukin 1B converting enzyme 2 antibody MCH 4 antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa |
| Cellular Localization | Cytosol. |
| SwissProt ID | Q92851 |
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Application
Fig1: Western blot analysis of Caspase-10 on K562 cells lysates using anti-Caspase-10 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Caspase-10 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspase-10 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of K562 cells with Caspase-10 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | K562, human liver cancer tissue, human kidney tissue. |
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| Application Notes | WB:1:1,000-1:2,000 FC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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