| 存储条件 |
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| Product Name | Caspase-14 [JJ089-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apoptosis related cysteine protease antibody CASP 14 antibody CASP-14 antibody CASP14 antibody Caspase 14 apoptosis related cysteine protease antibody Caspase 14 precursor antibody Caspase-14 subunit p10 antibody Caspase14 antibody CASPE_HUMAN antibody MGC119078 antibody MGC119079 antibody MICE antibody Mini ICE antibody |
| Product description | A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is composed of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6 and caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9, caspase-10, and caspase-14. Ced-3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. Caspase-14, also designated MICE (for mini-ICE), is highly expressed in embryonic tissues but appears to be absent from adult tissues. Procaspase-14 can be processed in vitro by caspase-8 and caspase-10 but not by other caspases. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Apoptosis related cysteine protease antibody CASP 14 antibody CASP-14 antibody CASP14 antibody Caspase 14 apoptosis related cysteine protease antibody Caspase 14 precursor antibody Caspase-14 subunit p10 antibody Caspase14 antibody CASPE_HUMAN antibody MGC119078 antibody MGC119079 antibody MICE antibody Mini ICE antibody |
|---|---|
| Molecular Weight(MW) | 28 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of Caspase-14 on MCF-7 cells lysates using anti-Caspase-14 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Caspase-14 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Caspase-14 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Caspase-14 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Caspase-14 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of A431 cells with Caspase-14 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | A431, MCF-7, HepG2, mouse skin tissue. |
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| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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