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| Product Name | SIRT6 [JJ20-69] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | 2810449N18Rik antibody AI043036 antibody Mono ADP ribosyltransferase sirtuin 6 antibody NAD-dependent protein deacetylase sirtuin-6 antibody Regulatory protein SIR2 homolog 6 antibody Regulatory protein SIR2 homolog antibody SIR2 like 6 antibody SIR2 like protein 6 antibody Sir2 related protein type 6 antibody SIR2-like protein 6 antibody SIR2L6 antibody SIR6_HUMAN antibody SIRT 6 antibody SIRT6 antibody Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody Sirtuin 6 antibody Sirtuin type 6 antibody Sirtuin6 antibody |
| Product description | The Silent Information Regulator (Sir2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of this family is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging. SirT6, a mammalian homolog of Sir2, is a nuclear, chromatin-associated protein that promotes the normal maintenance of genome integrity mediated by the base excision repair (BER) pathway. The BER pathway repairs single-stranded DNA lesions that arise spontaneously from endogenous alkylation, oxidation, and deamination events. SirT6 deficient mice show increased sensitivity to DNA-damaging agents, including the alkylating agents MMS and H2O2. In addition, these mice show genome instability with increased frequency of fragmented chromosomes, detached centromeres, and gaps. SirT6 may regulate the BER pathway by deacetylating DNA Polβ or other core components of the pathway. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFFC |
| WB:1:1,000 FC:1:50-1:100 ICC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | 2810449N18Rik antibody AI043036 antibody Mono ADP ribosyltransferase sirtuin 6 antibody NAD-dependent protein deacetylase sirtuin-6 antibody Regulatory protein SIR2 homolog 6 antibody Regulatory protein SIR2 homolog antibody SIR2 like 6 antibody SIR2 like protein 6 antibody Sir2 related protein type 6 antibody SIR2-like protein 6 antibody SIR2L6 antibody SIR6_HUMAN antibody SIRT 6 antibody SIRT6 antibody Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody Sirtuin 6 antibody Sirtuin type 6 antibody Sirtuin6 antibody |
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| Molecular Weight(MW) | 39 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q8N6T7 |
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Application
Fig1: ICC staining SIRT6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining SIRT6 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Flow cytometric analysis of Hela cells with SIRT6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | NIH/3T3, Hela. |
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| Application Notes | WB:1:1,000 FC:1:50-1:100 ICC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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