| 存储条件 |
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| Product Name | ATG4A [8C9C2] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | AI627006 antibody APG4 autophagy 4 homolog A antibody Apg4a antibody ATG4 autophagy related 4 homolog A (S. cerevisiae) antibody ATG4 autophagy related 4 homolog A antibody ATG4A antibody ATG4A_HUMAN antibody Atg4al antibody AUT like 2 cysteine endopeptidase antibody AUT-like 2 cysteine endopeptidase antibody Autl2 antibody Autophagin 2 antibody Autophagin-2 antibody Autophagy related 4A cysteine peptidase antibody Autophagy related cysteine endopeptidase 2 antibody Autophagy related protein 4 homolog A antibody Autophagy-related cysteine endopeptidase 2 antibody Autophagy-related protein 4 homolog A antibody AV169859 antibody Cysteine protease ATG4A antibody hAPG4A antibody MGC107179 antibody |
| Product description | Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. |
| Immunogen | Purified recombinant fragment of human ATG4A (AA: 258-398) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1：50-1:200 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | AI627006 antibody APG4 autophagy 4 homolog A antibody Apg4a antibody ATG4 autophagy related 4 homolog A (S. cerevisiae) antibody ATG4 autophagy related 4 homolog A antibody ATG4A antibody ATG4A_HUMAN antibody Atg4al antibody AUT like 2 cysteine endopeptidase antibody AUT-like 2 cysteine endopeptidase antibody Autl2 antibody Autophagin 2 antibody Autophagin-2 antibody Autophagy related 4A cysteine peptidase antibody Autophagy related cysteine endopeptidase 2 antibody Autophagy related protein 4 homolog A antibody Autophagy-related cysteine endopeptidase 2 antibody Autophagy-related protein 4 homolog A antibody AV169859 antibody Cysteine protease ATG4A antibody hAPG4A antibody MGC107179 antibody |
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| Molecular Weight(MW) | 45.4kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q8WYN0 |
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Application
Western blot analysis of ATG4A against human ATG4A (AA: 258-398) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-21 against HEK293 (1) and ATG4A (AA: 258-398)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunocytochemistry staining of ATG4A in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1711-21, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor
Application
Flow cytometric analysis of ATG4A was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-21, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells HL-60 cells |
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| Application Notes | WB:1:500-1:2,000 ICC:1：50-1:200 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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