AA589454 antibody Ancient ubiquitous protein 1 antibody AUP 1 antibody aup1 antibody AUP1_HUMAN antibody
Product description
The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR). Alternative splicing results in multiple transcript variants.
Immunogen
Purified recombinant fragment of human AUP1 (AA: 229-410) expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG2b
Host Species
Mouse
Tested Applications
WB
WB:1:500-1:2,000
Species Reactivity
HumanMouse
Concentration
1mg/mL
Target Information
Alternative Names
AA589454 antibody Ancient ubiquitous protein 1 antibody AUP 1 antibody aup1 antibody AUP1_HUMAN antibody
Western blot analysis of AUP1 against human AUP1 (AA: 229-410) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-17 against HEK293 (1) and AUP1 (AA: 229-410)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-17 against A431 (1), NIH/3T3 (2), Hela (3), SW480 (4), CHO3D10 (5), A549 (6), and SPC-A-1 (7) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Positive Control
A431, NIH/3T3, Hela, SW480, CHO3D10, A549, and SPC-A-1 cell
Application Notes
WB:1:500-1:2,000
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
