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| Product Name | CD209 [5C2A6] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | C type lectin domain family 4 member L antibody C-type lectin domain family 4 member L antibody CD 209 antibody CD209 antibody CD209 antigen antibody CD209 antigen-like protein A antibody CD209 molecule antibody CD209_HUMAN antibody Cd209a antibody CDSIGN antibody CIRE antibody CLEC4L antibody DC SIGN antibody DC SIGN1 antibody DC-SIGN antibody DC-SIGN1 antibody DCSIGN antibody Dendritic cell specific ICAM 3 grabbing nonintegrin 1 antibody Dendritic cell specific ICAM3 grabbing nonintegrin 1 antibody Dendritic cell-specific ICAM-3-grabbing non-integrin 1 antibody Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin antibody Dengue fever, protection against, included antibody Dentritic Cell Specific ICAM3 Grabbing Nonintegrin antibody HIV GP120 Binding Protein antibody MGC129965 antibody MGC130443 antibody SIGN-R1 antibody SIGNR5 antibody |
| Product description | This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants. |
| Immunogen | Purified recombinant fragment of human CD209 (AA: extra 270-404) expressed in E. Coli. |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | C type lectin domain family 4 member L antibody C-type lectin domain family 4 member L antibody CD 209 antibody CD209 antibody CD209 antigen antibody CD209 antigen-like protein A antibody CD209 molecule antibody CD209_HUMAN antibody Cd209a antibody CDSIGN antibody CIRE antibody CLEC4L antibody DC SIGN antibody DC SIGN1 antibody DC-SIGN antibody DC-SIGN1 antibody DCSIGN antibody Dendritic cell specific ICAM 3 grabbing nonintegrin 1 antibody Dendritic cell specific ICAM3 grabbing nonintegrin 1 antibody Dendritic cell-specific ICAM-3-grabbing non-integrin 1 antibody Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin antibody Dengue fever protection against included antibody Dentritic Cell Specific ICAM3 Grabbing Nonintegrin antibody HIV GP120 Binding Protein antibody MGC129965 antibody MGC130443 antibody SIGN-R1 antibody SIGNR5 antibody |
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| Molecular Weight(MW) | 45.8KD |
| Cellular Localization | Secreted and Cell membrane. |
| SwissProt ID | Q9NNX6 |
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Application
Western blot analysis of CD209 against human CD209 (AA: extra 270-404) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-59, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD209 against HEK293 (1) and CD209 (AA: extra 270-404)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-59, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD209 against Hela (1), U937 (2), THP-1 (3), HL-60 (4), and A431 (5) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-59, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD209 was done on Ramos cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-59, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela, U937, THP-1, HL-60, and A431 cell lysate, Ramos cells |
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| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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