| 存储条件 |
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| Product Name | GLRB [3B8A8] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | GAL 3R antibody GAL R3 antibody GAL3 R antibody GAL3-R antibody GAL3R antibody Galanin receptor 3 antibody Galanin receptor family member 3 antibody Galanin receptor type 3 antibody GALN R3 antibody GALNR 3 antibody GALNR3 antibody GALR 3 antibody GALR-3 antibody Galr3 antibody GALR3_HUMAN antibody OTTHUMP00000028820 antibody |
| Product description | This gene encodes the beta subunit of the glycine receptor, which is a pentamer composed of alpha and beta subunits. The receptor functions as a neurotransmitter-gated ion channel, which produces hyperpolarization via increased chloride conductance due to the binding of glycine to the receptor. Mutations in this gene cause startle disease, also known as hereditary hyperekplexia or congenital stiff-person syndrome, a disease characterized by muscular rigidity. Alternative splicing results in multiple transcript variants. |
| Immunogen | Purified recombinant fragment of human GLRB (AA: extra 23-160) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1：50-1:200 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | GAL 3R antibody GAL R3 antibody GAL3 R antibody GAL3-R antibody GAL3R antibody Galanin receptor 3 antibody Galanin receptor family member 3 antibody Galanin receptor type 3 antibody GALN R3 antibody GALNR 3 antibody GALNR3 antibody GALR 3 antibody GALR-3 antibody Galr3 antibody GALR3_HUMAN antibody OTTHUMP00000028820 antibody |
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| Molecular Weight(MW) | 56.1kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P48167 |
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Application
Western blot analysis of GLRB against human GLRB (AA: extra 23-160) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-32, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1710-32 against HEK293 (1) and GLRB (AA: extra 23-160)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-32, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunocytochemistry staining of GLRB in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1710-32, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor
Application
Flow cytometric analysis of GLRB was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-32, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells |
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| Application Notes | WB:1:500-1:2,000 ICC:1：50-1:200 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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