| 存储条件 |
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| Product Name | Caspase 5 [SD203-2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apoptosis related cysteine protease antibody CASP-5 antibody CASP5 antibody CASP5_HUMAN antibody Caspase-5 subunit p10 antibody ICE(rel)-III antibody ICERELIII antibody ICH 3 antibody ICH 3 protease antibody Protease ICH-3 antibody Protease TY antibody TY antibody TY protease antibody |
| Product description | Caspases are cysteine proteases which play important roles in the activation of cytokines and in apoptosis. The ICE subfamily of caspases comprises peptides closely related to caspase-1, which promotes maturation of interleukin 1β (IL-1β) and interleukin-18 (IL-18) by proteolytic cleavage of precursor forms to generate biologically active peptides. Both caspase-4 and caspase-5 are members of the caspase-1 subfamily, and are more closely related to each other than to other homologues. Caspase-5 (also designated ICErel-III, TY, ICH-3 and caspase-12 in mouse), can cleave its own precursor, an activity that requires the cysteine 245 residue. Frameshift mutations in caspase-5 have been identified in MMP tumors of the endometrium, colon, and stomach, indicating the caspase-5 may be a new target gene in the microsatellite mutator pathway for cancer. The human caspase 5 gene maps to chromosome 11q22.2-q22.3 and encodes a protein whose expression is barely detectable in most tissues except brain, with highest expression levels being found in lung, liver and skeletal muscle. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Apoptosis related cysteine protease antibody CASP-5 antibody CASP5 antibody CASP5_HUMAN antibody Caspase-5 subunit p10 antibody ICE(rel)-III antibody ICERELIII antibody ICH 3 antibody ICH 3 protease antibody Protease ICH-3 antibody Protease TY antibody TY antibody TY protease antibody |
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| Molecular Weight(MW) | 47 kDa |
| SwissProt ID | P51878 |
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Application
Fig1: ICC staining Caspase 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Caspase 5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Caspase 5 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Caspase 5 antibody. Counter stained with hematoxylin.| Positive Control | A549, SHG-44, Hela, human liver tissue. |
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| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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