| 存储条件 |
|---|
| Product Name | Cyclin A2 [SD2052] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCN1 antibody CCNA antibody Ccna2 antibody CCNA2_HUMAN antibody Cyclin A2 antibody Cyclin-A antibody Cyclin-A2 antibody |
| Product description | The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin- dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CCN1 antibody CCNA antibody Ccna2 antibody CCNA2_HUMAN antibody Cyclin A2 antibody Cyclin-A antibody Cyclin-A2 antibody |
|---|---|
| Molecular Weight(MW) | 49 kDa |
| Cellular Localization | Nucleus, Cytoplasm. |
Application
Fig1: Western blot analysis of Cyclin A2 on different lysates using anti-Cyclin A2 antibody at 1/1,000 dilution. Positive control: Lane 1: PC12 Lane 2: NIH/3T3
Application
Fig2: ICC staining Cyclin A2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cyclin A2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Cyclin A2 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.| Positive Control | RH-35, NIH/3T3, Hela, HepG2, human tonsil tissue, mouse colon tissue, human colon cancer tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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