| 存储条件 |
|---|
| Product Name | MTHFR [F3-D5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5 10 methylenetetrahydrofolate reductase (NADPH) antibody 5 10 methylenetetrahydrofolate reductase antibody Methylenetetrahydrofolate reductase (NAD(P)H) antibody Methylenetetrahydrofolate reductase antibody Methylenetetrahydrofolate reductase intermediate form antibody MTHFR antibody MTHR_HUMAN antibody |
| Product description | 5,10-methylenetetrahydrofolate reductase (MTHFR) is an essential enzyme in the folate-dependent regulation of methionine and homocysteine concentrations. MTHFR catalyzes the reduction of 5,10-methylenetetrahydrofolate (methyleneTHF) to 5- methyltetrahydrofolate (methylTHF). MethylTHF, the predominate form of circulating folate, is the principle carbon donor for homocysteine methylation, a reaction that yields methionine. Folate prevents neural tube defects, and can lower homocysteine levels, suggesting that MTHFR function is important in preventing cardiovascular disease. Mutations at the MTHFR gene may influence vascular changes, predisposition to leukemia, coronary artery disease and hyperhomocysteinemia. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 IHC:1:50-1:200 | |
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | 5 10 methylenetetrahydrofolate reductase (NADPH) antibody 5 10 methylenetetrahydrofolate reductase antibody Methylenetetrahydrofolate reductase (NAD(P)H) antibody Methylenetetrahydrofolate reductase antibody Methylenetetrahydrofolate reductase intermediate form antibody MTHFR antibody MTHR_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 75 kDa |
| Cellular Localization | Cytosol. |
| SwissProt ID | P42898 |
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Application
Fig1: Western blot analysis of MTHFR on rat heart tissue lysate using anti-MTHFR antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of MTHFR on HEK293 (1) and MTHFR-hIgGFc transfected HEK293 (2) cell lysate using anti-MTHFR antibody at 1/1,000 dilution.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MTHFR antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human intima cancer tissue using anti-MTHFR antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-MTHFR antibody. Counter stained with hematoxylin.| Positive Control | Rat heart tissue lysate, MTHFR-hIgGFc transfected HEK293 cell lysate, human lung cancer tissue, human intima cancer tissue, human prostate tissue. |
|---|---|
| Application Notes | WB:1:500 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
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