| 存储条件 |
|---|
| Product Name | HAS3 [B2-C9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HA synthase 3 antibody Has3 antibody HAS3_HUMAN antibody Hyaluronan synthase 3 antibody Hyaluronate synthase 3 antibody Hyaluronic acid synthase 3 antibody |
| Product description | The extracellular matrix in most vertebrates express HA, which is a high molecular weight linear polysaccharide composed of alternating glucuronic acid and N-acetylglucosamine residues linked by b-1,3 and b-1,4 glycosidic bonds. HAS3 produces lower molecular weight HA. The expression of the three HAS isoforms is more prominent in growing cells than in resting cells and is differentially regulated by various stimuli, suggesting distinct functional roles of the three proteins. HAS3 produces both secreted and cell-associated forms of hyaluronan and is the most active of the three isoforms of this enzyme in adults. HAS3 gene expression plays a crucial role in the regulation of hyaluronan synthesis in the epidermis. Specifically, IFN-g markedly upregulates HAS3 mRNA, whereas TGFβ downregulates HAS3 transcript levels. The human HAS3 gene maps to chromosome 16q22.1. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:100-1:500 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | HA synthase 3 antibody Has3 antibody HAS3_HUMAN antibody Hyaluronan synthase 3 antibody Hyaluronate synthase 3 antibody Hyaluronic acid synthase 3 antibody |
|---|---|
| Molecular Weight(MW) | 63 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | O00219 |
|---|
Application
Fig1: Western blot analysis of HAS3 on human HAS3 recombinant protein using anti-HAS3 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-HAS3 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-HAS3 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of HeLa cells with HAS3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human HAS3 recombinant protein, human cervical cancer tissue, human prostate tissue, HeLa. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
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