| 存储条件 |
|---|
| Product Name | TGFb1 [B2-F6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The transforming growth factors b (TGFβ) superfamily is composed of numerous growth and differentiation factors, including TGFβ1-3. TGFβs are secreted from cells as latent complexes consisting of mature dimeric growth factor, the latency-associated propeptide (LAP) and a distinct gene product, latent TGFβ binding protein LTBP. Members of the TGF superfamily are involved in embryonic development and adult tissue homeostasis. The precursor of TGFβ is cleaved into mature TGFβ and LAP (latency associated peptide). Mature TGFβ remains associated with LAP by non-covalent interactions that block TGFβ from binding to its receptor. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:100-1:500 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Molecular Weight(MW) | 44 kDa |
|---|---|
| Cellular Localization | Secreted. Extracellular matrix. |
| SwissProt ID | P01137 |
|---|
Application
Fig1: Western blot analysis of TGF beta 1 on human TGF beta 1 recombinant protein using anti- TGF beta 1 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti- TGF beta 1 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lymphoid tissue using anti- TGF beta 1 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of A549 cells with TGF beta 1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human TGF beta 1 recombinant protein, human lung tissue, human lymphoid tissue, A549. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
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