| 存储条件 |
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| Product Name | KDM4A [E7-H1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JHDM3A antibody JmjC domain containing histone demethylation protein 3A antibody JmjC domain-containing histone demethylation protein 3A antibody JMJD2 antibody JMJD2A antibody jumonji C domain containing histone demethylase 3A antibody Jumonji domain containing 2 antibody Jumonji domain containing 2A antibody Jumonji domain containing protein 2A antibody Jumonji domain-containing protein 2A antibody KDM4A antibody KDM4A_HUMAN antibody KIAA0677 antibody Lysine (K) specific demethylase 4A antibody Lysine-specific demethylase 4A antibody TDRD14A antibody Tudor domain containing 14A antibody |
| Product description | JMJD2A (jumonji domain containing 2A), also designated jumonji C domain-containing histone demethylation protein 3A, is a 1,064 amino acid protein encoded by the human gene JMJD2A. JMJD2A belongs to the JHDM3 histone demethylase family and contains one JmjC domain, one JmjN domain, two PHD-type zinc fingers and two Tudor domains. JMJD2A is histone de-methylase that specifically demethylates Lys 9 and Lys 36 residues of Histone H3, thereby playing a central role in histone code. It does not demethylate histone H3 Lys 4, H3 Lys 27 nor H4 Lys 20, however, it will demethylate tri-methylated H3 Lys 9 and H3 Lys 36 residue, while it has no activity on mono- and dimethylated residues. JMJD2A demethylation of lysine residues will generate formaldehyde and succinate. It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. JMJD2A is a ubiquitously expressed nuclear protein. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Purification | Unpurified |
| Alternative Names | JHDM3A antibody JmjC domain containing histone demethylation protein 3A antibody JmjC domain-containing histone demethylation protein 3A antibody JMJD2 antibody JMJD2A antibody jumonji C domain containing histone demethylase 3A antibody Jumonji domain containing 2 antibody Jumonji domain containing 2A antibody Jumonji domain containing protein 2A antibody Jumonji domain-containing protein 2A antibody KDM4A antibody KDM4A_HUMAN antibody KIAA0677 antibody Lysine (K) specific demethylase 4A antibody Lysine-specific demethylase 4A antibody TDRD14A antibody Tudor domain containing 14A antibody |
|---|---|
| Molecular Weight(MW) | 120 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | O75164 |
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Application
Fig1: Western blot analysis of KDM4A on HEK293 (1) and KDM4A-hIgGFc transfected HEK293 (2) cell lysate using anti-KDM4A antibody at 1/1,000 dilution.
Application
Fig2: ICC staining KDM4A (green) and actin filaments (red) in NTERA-2 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human larynx cancer tissue using anti-KDM4A antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-KDM4A antibody. Counter stained with hematoxylin.| Positive Control | KDM4A-hIgGFc transfected HEK293 cell lysate, NTERA-2, human larynx cancer tissue, human colon cancer tissue. |
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| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
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