Thrombopoietin (TPO or THPO), also known as c-Mpl ligand (c-Mpl L), is a cytokine that plays a central role in megakaryopoiesis by influencing the development and maturation of megakaryocytes and platelet production. TPO is expressed by both mature and progenitor megakaryocytes, as well as by human platelets. Human TPO cDNA encodes a 332 amino acid precursor with a 21 amino acid signal peptide which is cleaved to generate the mature protein. TPO isolated from serum ranges in molecular weight, which suggests that the protein is highly glycosylated. TPO exerts its biological effects through the TPO receptor, c-Mpl. Stimulation of c-Mpl with TPO results in the activation of the Janus tyrosine kinase family members, The gene encoding TPO maps to human chromosome 3q26.3-q27.
Fig1: Western blot analysis of Thrombopoietin on human Thrombopoietin recombinant protein using anti- Thrombopoietin antibody at 1/1,000 dilution.
Application
Fig2: Flow cytometric analysis of MCF-7 cells with Thrombopoietin antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Positive Control
Human THPO recombinant protein, MCF-7.
Application Notes
WB:1:500-1:1,000 FC:1:100-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
