AI467657 antibody lu antibody PLZF antibody Promyelocytic leukemia zinc finger protein antibody ZBT16_HUMAN antibody ZBTB16 antibody Zfp145 antibody Zinc finger and BTB domain containing 16 antibody zinc finger and BTB domain containing protein 16 antibody Zinc finger and BTB domain-containing protein 16 antibody Zinc finger protein 145 (Kruppel like expressed in promyelocytic leukemia) antibody Zinc finger protein 145 antibody Zinc finger protein PLZF antibody Znf145 antibody
Product description
Hypermethylated in cancer (HIC-1) was originally identified as a target of p53-induced gene expression. HIC-1 is deleted in the genetic disorder Miller-Dieker syndrome (MDS). The expression of HIC-1 is also frequently suppressed in leu-kemia and other various cancers due to the hypermethylation of specific DNA regions and the resulting transcriptional silencing. These and other studies indicate that HIC-1 acts as a putative tumor suppressor protein that mediates transcriptional repression. HIC-1 is ubiquitously expressed in adult tissues and its structure is defined by five zinc fingers and an N-terminal broad-complex POZ (or BTB) domain. The BTB/POZ domain mediates homomeric and heteromeric POZ-POZ interactions and is common to transcriptional regulators involved in chromatin modeling. In several BTB/POZ containing proteins, including BCL-6 and the promyelocytic leukemia zinc finger (PLZF) oncoprotein, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and is directly involved in repressing and silencing gene transcription. When this domain is deleted, as with the oncogenic PLZF-RAR chimera of promyelocytic leukemias, this transcriptional repression is attenuated. Conversely, HIC-1 does not interact with components of the HDAC complex, suggesting that HIC-1-induced transcriptional repression is unassociated with the POZ/BTB domain.
Immunogen
Recombinant protein
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WBICC
WB:1:500-1:2,000 ICC:1:50-1:200
Species Reactivity
Human
Concentration
2mg/ml
Target Information
Alternative Names
AI467657 antibody lu antibody PLZF antibody Promyelocytic leukemia zinc finger protein antibody ZBT16_HUMAN antibody ZBTB16 antibody Zfp145 antibody Zinc finger and BTB domain containing 16 antibody zinc finger and BTB domain containing protein 16 antibody Zinc finger and BTB domain-containing protein 16 antibody Zinc finger protein 145 (Kruppel like expressed in promyelocytic leukemia) antibody Zinc finger protein 145 antibody Zinc finger protein PLZF antibody Znf145 antibody
Fig1: Western blot analysis of ZBTB16 on Hela using anti-ZBTB16 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining ZBTB16 (green) and actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Positive Control
Hela.
Application Notes
WB:1:500-1:2,000 ICC:1:50-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
