In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family. The PP1 family is comprised of subfamily members PP1α, PP1β and PP1γ, which are MgATP-dependent enzymes. PP1 inactivity is maintained through its association with the inhibitory protein NIPP-1 (nuclear inhibitor of PP1). Phosphorylation of NIPP-1 by cAMP-PK or casein kinase II results in the release of active PP1.
Fig1: Western blot analysis of PPP1CA on human PPP1CA recombinant protein using anti-PPP1CA antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PPP1CA on different cell lysates using anti-PPP1CA antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Hela Lane 3: HepG2 Lane 4: CF-7 Lane 5:Jurkat? Lane 6: A549
Positive Control
Human PPP1CA recombinant protein, A431, Hela, HepG2, MCF-7, Jurkat, A549.
Application Notes
WB:1:500
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
