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| Product Name | MEF2C [G2-H2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C5DELq14.3 antibody DEL5q14.3 antibody MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) antibody MADS box transcription enhancer factor 2, polypeptide C antibody MEF2C antibody MEF2C_HUMAN antibody Myocyte enhancer factor 2C antibody Myocyte specific enhancer factor 2C antibody Myocyte-specific enhancer factor 2C antibody OTTHUMP00000222409 antibody Similar to MADS box transcription enhancer factor 2 polypeptide C antibody |
| Product description | The myocyte enhancer factor-2 (MEF-2) family of transcription factors associate with co-repessors or co-activators to regulate development and function of T cells, neuronal cells, and muscle cells. Four family members arise from alternatively spliced transcripts, termed MEF-2A, -2B, -2C, and -2D. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each duing embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5, and MRF4, are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF-2) family. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | C5DELq14.3 antibody DEL5q14.3 antibody MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) antibody MADS box transcription enhancer factor 2 polypeptide C antibody MEF2C antibody MEF2C_HUMAN antibody Myocyte enhancer factor 2C antibody Myocyte specific enhancer factor 2C antibody Myocyte-specific enhancer factor 2C antibody OTTHUMP00000222409 antibody Similar to MADS box transcription enhancer factor 2 polypeptide C antibody |
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| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q06413 |
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Application
Fig1: Western blot analysis of MEF2C on human MEF2C recombinant protein using anti-MEF2C antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of MEF2C on HEK293 (1) and MEF2C-hIgGFc transfected HEK293 (2) cell lysate using anti-MEF2C antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of MEF2C on NIH3T3 (1) and 3T3-L1 (2) cell lysate using anti-MEF2C antibody at 1/1,000 dilution.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MEF2C antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-MEF2C antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with MEF2C antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Human MEF2C recombinant protein, MEF2C-hIgGFc transfected HEK293 cell lysate, NIH3T3, 3T3-L1 cell lysate, human colon cancer tissue, human esophageal cancer tissue, Hela. |
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| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
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