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| Product Name | KEAP1 [B6-F10] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | Cytosolic inhibitor of Nrf2 antibody INrf 2 antibody INrf2 antibody Keap 1 antibody KEAP1 antibody KEAP1_HUMAN antibody Kelch like ECH associated protein 1 antibody Kelch like family member 19 antibody Kelch like protein 19 antibody Kelch-like ECH-associated protein 1 antibody Kelch-like protein 19 antibody KIAA0132 antibody KLHL 19 antibody KLHL19 antibody MGC10630 antibody MGC1114 antibody MGC20887 antibody MGC4407 antibody MGC9454 antibody |
| Product description | Keap1 (kelch-like ECH-associated protein 1, INrf2, KLHL19) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates Nrf2 (NF-E2-related factor 2). Steady state levels of proteins are under the influence of the ubiquitin pathway, which consists of ubiquitin activation (E1), conjugation (E2) and ligation (E3). Keap1 assembles into an E3 ubiquitin ligase complex with Cul3 and Rbx1 and targets lysine residues in the N-terminal Neh2 domain of Nrf2 for ubiquitin conjugation. The Keap1-Nrf2 system mediates cytoprotective gene expression in response to oxidative and/or electrophilic stresses. Keap1 constitutively suppresses Nrf2 activity under unstressed conditions, oxidants or electrophiles provoke the repression of Keap1 activity, inducing Nrf2 activation. Cys 273 and Cys 288 residues of Keap1 are required for suppressing Nrf2 nuclear accumulation. Keap1 sequesters Nrf2 in the cytoplasm through an active Crm1/exportin-dependent nuclear export mechanism. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:250~1:1000 ICC:1:100~1:200 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Cytosolic inhibitor of Nrf2 antibody INrf 2 antibody INrf2 antibody Keap 1 antibody KEAP1 antibody KEAP1_HUMAN antibody Kelch like ECH associated protein 1 antibody Kelch like family member 19 antibody Kelch like protein 19 antibody Kelch-like ECH-associated protein 1 antibody Kelch-like protein 19 antibody KIAA0132 antibody KLHL 19 antibody KLHL19 antibody MGC10630 antibody MGC1114 antibody MGC20887 antibody MGC4407 antibody MGC9454 antibody |
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| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. |
Application
Fig1: Western blot analysis of KEAP1 on human KEAP1 recombinant protein using anti-KEAP1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of KEAP1 on NIH3T3 (1) and A549 (2) cell lysate using anti-KEAP1 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining KEAP1 (green) and Actin filaments (red) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of HepG2 cells with KEAP1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Human KEAP1 recombinant protein, NIH3T3, A549 cell lysate, HeLa, HepG2. |
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| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
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