| 存储条件 |
|---|
| Product Name | CBX8 [D4-C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cbx8 antibody CBX8_HUMAN antibody Chromobox Homolog 8 (Drosophila Pc Class) antibody Chromobox Homolog 8 (Pc Class Homolog, Drosophila) antibody Chromobox Homolog 8 antibody Chromobox protein homolog 8 antibody hPc3 antibody Pc Class 3 Homolog (Drosophila) antibody Pc Class 3 Homolog antibody Pc3 antibody Polycomb 3 antibody Polycomb 3 homolog antibody RC1 antibody Rectachrome 1 antibody |
| Product description | Polycomb group (PcG) proteins form multiprotein complexes and play a role in gene silencing and Hox gene regulation by altering chromatin structure during transcription. CBX4 (chromobox homolog 4) and CBX8 (chromobox homolog 8), also known as PC2 or NBP16 and PC3 or RC1, respectively, are PcG proteins that show structural similarity to M33 and, like M33, bind the PcG protein RING1 through a conserved c-box motif located in the C-terminus of RING1. However, CBX8 has only been shown to bind RING1 in vivo with covalently modified forms of RING1. CBX8 also interacts with the carboxy-terminus of AF9, a transcriptional activator implicated in the development of acute lukemias. CBX8 acts as a long range transcriptional silencer when targeted to a reporter gene by a heterologous DNA-binding domain. The human MPc3 gene maps to chromosome 17q25.3 and encodes a 389 amino acid protein. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:250~1:1000 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Cbx8 antibody CBX8_HUMAN antibody Chromobox Homolog 8 (Drosophila Pc Class) antibody Chromobox Homolog 8 (Pc Class Homolog Drosophila) antibody Chromobox Homolog 8 antibody Chromobox protein homolog 8 antibody hPc3 antibody Pc Class 3 Homolog (Drosophila) antibody Pc Class 3 Homolog antibody Pc3 antibody Polycomb 3 antibody Polycomb 3 homolog antibody RC1 antibody Rectachrome 1 antibody |
|---|---|
| Molecular Weight(MW) | 43 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9HC52 |
|---|
Application
Fig1: Western blot analysis of CBX8 on human CBX8 recombinant protein using anti-CBX8 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CBX8 on HEK293 (1) and CBX8-hIgGFc transfected HEK293 (2) cell lysate using anti-CBX8 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of Hela cells with CBX8 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CBX8 recombinant protein, CBX8-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:250~1:1000 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
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