The thrombospondin proteins (TSP 1-4) compose a family of glycoproteins that are involved in cell-to-cell and cell-to-matrix signaling. These extracellular, cell-surface proteins form complexes of both homo- and heteromultimers. Thrombospondins play a role in development, aggregation of platelets, adhesion and migration of cells, and progression of cells through the growth cycle. Thrombospondin 1 is released from platelets in response to Thrombin stimulation and is a transient component of the extracellular matrix of developing and repairing tissues. Thrombospondin 2 shares a high degree of homology with Thrombospondin 1 and is thought to have overlapping but unique functions. Thrombospondin 3 is a developmentally regulated heparin binding protein. Thrombospondin 4 is neuronally expressed and stimulates neurite outgrowth.
Fig1: Western blot analysis of THBS1 on human THBS1 recombinant protein using anti-THBS1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of THBS1 on HEK293 (1) and THBS1-hIgGFc transfected HEK293 (2) cell lysate using anti-THBS1 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of Hela cells with THBS1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Positive Control
Human THBS1 recombinant protein, THBS1-hIgGFc transfected HEK293 cell lysate, Hela.
Application Notes
WB:1:500-1:2,000 FC:1:50-1:100
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
