| 存储条件 |
|---|
| Product Name | DOC2-DAB2 [H7-C4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Doc2 antibody Doc2-alpha antibody Doc2a antibody DOC2A_HUMAN antibody Double C2 domain alpha antibody Double C2 like domains, alpha antibody Double C2-like domain-containing protein alpha antibody |
| Product description | DAB2 is a component of the CSF1 signal transduction pathway. DAB2 mRNA is expressed in normal ovarian epithelial cells but is down regulated or absent from ovarian carcinoma cell lines. The down regulation of DAB2 may play an important role in ovarian carcinogenesis. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2 like domains, alpha and beta). |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Doc2 antibody Doc2-alpha antibody Doc2a antibody DOC2A_HUMAN antibody Double C2 domain alpha antibody Double C2 like domains alpha antibody Double C2-like domain-containing protein alpha antibody |
|---|---|
| Molecular Weight(MW) | 82 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P98082 |
|---|
Application
Fig1: Western blot analysis of DAB2 on human DAB2 recombinant protein using anti- DAB2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of DAB2 on HEK293 (1) and DAB2-hIgGFc transfected HEK293 (2) cell lysate using anti- DAB2 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining DAB2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of Hela cells with DAB2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human DAB2 recombinant protein, DAB2-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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