The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. RIP4, also known as RIPK4 (receptor-interacting serine/threonine-protein kinase 4), PKK (PKC δ-interacting protein kinase), DIK, ANKK2 or ANKRD3 (ankyrin repeat domain-containing protein 3), is a peripheral membrane protein that belongs to the TKL Ser/Thr protein kinase family and functions as a receptor-interacting protein (RIP) that modulates epidermal growth and differentiation. RIPs interact with the intracellular domain of tumor necrosis factor receptors (TNFRs) and facilitate downstream signaling and apoptosis induction. RIP4 contains ten ankyrin-repeats and one protein kinase domain, and participates in the activation of NFκB. Two isoforms exist due to alternative splicing events.
Fig1: Western blot analysis of RIPK4 on?human RIPK4 recombinant protein using anti-RIPK4 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RIPK4 on HEK293 (1) and RIPK4-hIgGFc transfected HEK293 (2) cell lysate using anti-RIPK4 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of SK-N-SH cells with RIPK4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Positive Control
Human RIPK4 recombinant protein, RIPK4-hIgGFc transfected HEK293 cell lysate, SK-N-SH.
Application Notes
WB:1:500-1:2,000 FC:1:100-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
