| 存储条件 |
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| Product Name | PGRMC1 [G8-G7] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | HPR6.6 antibody Membrane associated progesterone receptor component 1 antibody Membrane-associated progesterone receptor component 1 antibody mPR antibody PGRC1_HUMAN antibody PGRMC antibody Pgrmc1 antibody Progesterone binding protein antibody Progesterone receptor membrane component 1 antibody |
| Product description | PGRMC1 (progesterone receptor membrane component 1), also known as MPR, is a 195 amino acid single-pass membrane protein that localizes to both the endoplasmic reticulum and to the microsome and contains one cytochrome b5 heme-binding domain. Expressed in a variety of tissues with highest expression in kidney and liver, PGRMC1 functions as a receptor for progesterone, a steroid hormone that is involved in embryonic development and is crucial for proper female maturation. The gene encoding PGRMC1 maps to human chromosome X, which contains nearly 153 million base pairs and houses over 1,000 genes. In conjunction with chromosome Y, chromosome X is responsible for sex determination, as an X and a Y chromosome lead to normal male development, while two copies of an X chromosome lead to normal female development. There are a number of conditions related to an abnormal number and combination of sex chromosomes, some of which include Turner's syndrome, color blindness, hemophilia and Duchenne muscular dystrophy |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | HPR6.6 antibody Membrane associated progesterone receptor component 1 antibody Membrane-associated progesterone receptor component 1 antibody mPR antibody PGRC1_HUMAN antibody PGRMC antibody Pgrmc1 antibody Progesterone binding protein antibody Progesterone receptor membrane component 1 antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Microsome membrane. Endoplasmic reticulum membrane. |
| SwissProt ID | O00264 |
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Application
Fig1: Western blot analysis of PGRMC1 on human PGRMC1 recombinant protein using anti-PGRMC1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PGRMC1 on HEK293 (1) and PGRMC1-hIgGFc transfected HEK293 (2) cell lysate using anti-PGRMC1 antibody at 1/1,000 dilution.
Application
Fig3:ICC staining PGRMC1 (green) and Actin filaments (red) in SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-PGRMC1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human ?breast cancer tissue using anti-PGRMC1 antibody. Counter stained with hematoxylin.
Application
Fig6:Flow cytometric analysis of A549 cells with PGRMC1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PGRMC1 recombinant protein, PGRMC1-hIgGFc transfected HEK293 cell lysate, SK-OV-3, A549, human cervical cancer tissue, human ?breast cancer tissue. |
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| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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