| 存储条件 |
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| Product Name | ARF1 [E9-D8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADP Ribosylation Factor 1 antibody ADP-ribosylation factor 1 antibody ARF 1 antibody ARF1 antibody ARF1_HUMAN antibody |
| Product description | ADP-ribosylation factors (ARFs), are small guanine nucleotide-binding proteins that enhance the enzymatic activities of cholera toxin, and constitute one family of the RAS superfamily. ARFs are essential and ubiquitous in eukaryotes, as they are involved in vesicular transport and functioning via phospholipase D activation. ARF proteins play a role in membrane traffic and organelle integrity and are intimately tied to their reversible association with membranes and distinct interactions with membrane phospholipids. ARF1 is regulated by the binding and hydrolysis of GTP. Coatomer, or COPI, is a heptameric protein recruited to membranes by ARF1. Research demonstrates that guanine nucleotide exchange-activated ARF1, when located at the Golgi membrane, recruits and binds cytoplasmic COPI to the membranes. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | ADP Ribosylation Factor 1 antibody ADP-ribosylation factor 1 antibody ARF 1 antibody ARF1 antibody ARF1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Golgi apparatus. Cytoplasm |
| SwissProt ID | P84077 |
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Application
Fig1: Western blot analysis of ARF1 on human ARF1 recombinant protein using anti-ARF1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ARF1 on HEK293 (1) and ARF1-hIgGFc transfected HEK293 (2) cell lysate using anti-ARF1 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining ARF1 (green) and Actin filaments (red) in HL-7702 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:ICC staining ARF1 (green) and Actin filaments (red) in SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-ARF1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-ARF1 antibody. Counter stained with hematoxylin.
Application
Fig7:Flow cytometric analysis of Hela cells with ARF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | human ARF1 recombinant protein, ARF1-hIgGFc transfected HEK293 cell lysate, HL-7702, SK-OV-3, Hela, human stomach cancer tissue, human bladder cancer tissue. |
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| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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