Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110δ expression is restricted to white blood cells. It has been shown to bind p85α and β, but it apparently does not phosphorylate these subunits. p110δ seems to have the capacity to autophosphorylate. p110γ does not interact with the p85 subunits. It has been shown to be activated by α and βγ heterotrimeric G proteins.
Fig1: Western blot analysis of PIK3CG on human PIK3CG recombinant protein using anti-PIK3CG antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PIK3CG on HEK293 (1) and PIK3CG-hIgGFc transfected HEK293 (2) cell lysate using anti- PIK3CG antibody at 1/1,000 dilution.
Positive Control
Human PIK3CG recombinant protein, PIK3CG-hIgGFc transfected HEK293 cell lysate,
Application Notes
WB:1:500-1:2,000
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
