| 存储条件 |
|---|
| Product Name | KDM4A [G4-E10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JHDM3A antibody JmjC domain containing histone demethylation protein 3A antibody JmjC domain-containing histone demethylation protein 3A antibody JMJD2 antibody JMJD2A antibody jumonji C domain containing histone demethylase 3A antibody Jumonji domain containing 2 antibody Jumonji domain containing 2A antibody Jumonji domain containing protein 2A antibody Jumonji domain-containing protein 2A antibody KDM4A antibody KDM4A_HUMAN antibody KIAA0677 antibody Lysine (K) specific demethylase 4A antibody Lysine-specific demethylase 4A antibody TDRD14A antibody Tudor domain containing 14A antibody |
| Product description | Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. Isoform 2: Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Purification | Unpurified |
| Alternative Names | JHDM3A antibody JmjC domain containing histone demethylation protein 3A antibody JmjC domain-containing histone demethylation protein 3A antibody JMJD2 antibody JMJD2A antibody jumonji C domain containing histone demethylase 3A antibody Jumonji domain containing 2 antibody Jumonji domain containing 2A antibody Jumonji domain containing protein 2A antibody Jumonji domain-containing protein 2A antibody KDM4A antibody KDM4A_HUMAN antibody KIAA0677 antibody Lysine (K) specific demethylase 4A antibody Lysine-specific demethylase 4A antibody TDRD14A antibody Tudor domain containing 14A antibody |
|---|---|
| Molecular Weight(MW) | 121 kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | O75164 |
|---|
Application
Fig1: Western blot analysis of KDM4A on human KDM4A recombinant protein using anti-KDM4A antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of KDM4A on HEK293 (1) and KDM4A-hIgGFc transfected HEK293 (2) cell lysate using anti-KDM4A antibody at 1/1,000 dilution.
Application
Fig3: ICC staining KDM4A (green) and Actin filaments (red) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | Human KDM4A recombinant protein, KDM4A-hIgGFc transfected HEK293 cell lysate, Hela |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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