| 存储条件 |
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| Product Name | TIE1 [D2-D12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JKT 14 antibody JTK14 antibody TIE antibody Tie1 antibody TIE1_HUMAN antibody Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains antibody Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody Tyrosine protein kinase receptor Tie 1 antibody Tyrosine-protein kinase receptor Tie-1 antibody |
| Product description | Receptor tyrosine kinases play key roles in signal transduction across cell surfaces in biological systems, including the vascular system. These receptors comprise a large and diverse family of catalytically related proteins that, on the basis of sequence and structural similarities, can be divided into several different evolutionary subfamilies. The cloning and characterization of Tie-1 (also designated Tie), a novel human endothelial cell surface receptor tyrosine kinase, has been reported. The extracellular domain of the predicted Tie-1 protein product has an unusual multidomain structure consisting of a cluster of three epidermal growth factor homology motifs localized between two immunoglobulin-like loops, which are followed by three fibronectin type III repeats next to the transmembrane region. An additional member of this family has been identified as Tie-2 (also designated Tek). Tie-1 and Tie-2 have been shown to be encoded by distinct genes and to represent members of a new class of receptor tyrosine kinases. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500-1:2,000 IHC:1:200-1:1,000 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Purification | Unpurified |
| Alternative Names | JKT 14 antibody JTK14 antibody TIE antibody Tie1 antibody TIE1_HUMAN antibody Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains antibody Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody Tyrosine protein kinase receptor Tie 1 antibody Tyrosine-protein kinase receptor Tie-1 antibody |
|---|---|
| Molecular Weight(MW) | 125 kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P35590 |
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Application
Fig1: Western blot analysis of TIE1 on human TIE1 recombinant protein using anti-TIE1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of TIE1 on HEK293 (1) and RAN-hIgGFc transfected HEK293 (2) cell lysate using anti-TIE1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of TIE1 on HepG2 cell lysate using anti-TIE1 antibody at 1/1,000 dilution.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-CD68 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD68 antibody. Counter stained with hematoxylin.| Positive Control | Human TIE1 recombinant protein, TIE1-hIgGFc transfected HEK293 cell lysate, HepG2, human ovarian cancer tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:200-1:1,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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