etylcholine and glutamate receptors, both of which are composed of five homologous subunits forming a transmembrane aqueous pore. These transmembrane receptors change conformation in response to their cognate neurotransmitter. Nicotinic acetylcholine receptors are found at the postsynaptic membrane of the neuromuscular junction and bind acetylcholine molecules, allowing ions to move through the pore. Glutamate receptors are found in the postsynaptic membrane of cells in the central nervous system. The activity that is generated at the synapse by the binding of acetylcholine is terminated by acetylcholinesterase, an enzyme that rapidly hydrolyzes acetylcholine. AChRα4, also known as EBN, BFNC, EBN1, NACHR, NACRA4, NACHRA4 or CHRNA4, is a 627 amino acid multi-pass membrane protein associated with nocturnal frontal lobe epilepsy type 1 (ENFL1), an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
Fig1: Western blot analysis of CHRNA4 on human CHRNA4 recombinant protein using anti-CHRNA4 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CHRNA4 on HEK293 (1) and CHRNA4-hIgGFc transfected HEK293 (2) cell lysate using anti-CHRNA4 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of SH-SY5Y cells with CHRNA4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Positive Control
Human CHRNA4 recombinant protein, CHRNA4-hIgGFc transfected HEK293 cell lysate, SH-SY5Y.
Application Notes
WB:1:500-1:2,000 FC:1:50-1:100
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
