130 kDa retinoblastoma associated protein antibody FLJ26459 antibody p130 antibody PRB 2 antibody PRB2 antibody Rb 2 antibody Rb2 antibody RBL 2 antibody RBL2 antibody RBR 2 antibody RBR2 antibody Retinoblastoma like 2 antibody Retinoblastoma like protein 2 antibody Retinoblastoma related gene antibody
Product description
The human retinoblastoma gene product Rb plays an important role in the negative regulation of cell proliferation. The Rb family includes p107 and p130, which form complexes with E2F proteins, and share a high degree of structural homology in the adenovirus E1A binding domain (i.e., "pocket region"), which plays a primary role in the function of these proteins. The Rb family members undergo cell cycle dependent phosphorylation during mid-G1 to S phase transition, which is dependent upon the activity of cyclin D/cdk4. In contrast to pRb and p107, p130 is also phosphorylated during G0 and the early G1 phase of the cell cycle. p130 is specifically phosphorylated on serine and threonine residues in cells arrested in G0 by serum deprivation or density arrest, and these residues are clustered within a short co-linear region unique to p130 defined as the Loop.
Immunogen
Recombinant protein
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WBFC
WB:1:500-1:2,000 FC:1:50-1:100
Species Reactivity
Human
Concentration
2mg/ml
Target Information
Alternative Names
130 kDa retinoblastoma associated protein antibody FLJ26459 antibody p130 antibody PRB 2 antibody PRB2 antibody Rb 2 antibody Rb2 antibody RBL 2 antibody RBL2 antibody RBR 2 antibody RBR2 antibody Retinoblastoma like 2 antibody Retinoblastoma like protein 2 antibody Retinoblastoma related gene antibody
Fig1: Western blot analysis of RBL2 on human RBL2 recombinant protein using anti-RBL2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RBL2 on HEK293 (1) and RBL2-hIgGFc transfected HEK293 (2) cell lysate using anti-RBL2 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of Hela cells with RBL2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Positive Control
Human RBL2 recombinant protein, RBL2-hIgGFc transfected HEK293 cell lysate, Hela.
Application Notes
WB:1:500-1:2,000 FC:1:50-1:100
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
