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| Product Name | PDPK1 [G11-E4] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | 3 phosphoinositide dependent protein kinase 1 antibody 3-phosphoinositide-dependent protein kinase 1 antibody hPDK 1 antibody hPDK1 antibody MGC20087 antibody MGC35290 antibody OTTHUMP00000159109 antibody OTTHUMP00000159110 antibody OTTHUMP00000174525 antibody PDK1 antibody Pdpk1 antibody PDPK1_HUMAN antibody PDPK2 antibody PDPK2P antibody PkB kinase antibody PkB kinase like gene 1 antibody PkB like 1 antibody PRO0461 antibody Protein kinase antibody |
| Product description | PDPK1 (3-phosphoinositide dependent protein kinase 1), also known as PDK1, PDPK2, PDPK2P or PRO0461, is 556 amino acid ubiquitously expressed protein that localizes to the cell membrane, cytoplasm and nucleus. Acting as a master kinase, PDPK1 phosphorylates and activates a subgroup of the AGC family of protein kinases. PDPK1 is involved in mediating signal transduction for controlling proliferation, survival, and growth of developing pancreatic beta cells, regulating Ca2+ uptake and Ca2+-activated K+ channels of mast cells, regulation of chemotaxis and motility of vascular endothelial cells, cardiac homeostasis, and thymocyte development. Belonging to the protein kinase superfamily, PDPK1 contains a PH domain, which play an essential role in homodimerization, localization and nuclear import of PDPK1, and a protein kinase domain. PDPK1 exists as five alternatively spliced isoforms and is encoded by a gene located on human chromosome 16p13.3. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 2mg/ml |
| Alternative Names | 3 phosphoinositide dependent protein kinase 1 antibody 3-phosphoinositide-dependent protein kinase 1 antibody hPDK 1 antibody hPDK1 antibody MGC20087 antibody MGC35290 antibody OTTHUMP00000159109 antibody OTTHUMP00000159110 antibody OTTHUMP00000174525 antibody PDK1 antibody Pdpk1 antibody PDPK1_HUMAN antibody PDPK2 antibody PDPK2P antibody PkB kinase antibody PkB kinase like gene 1 antibody PkB like 1 antibody PRO0461 antibody Protein kinase antibody |
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| Molecular Weight(MW) | 63 kDa |
| Cellular Localization | Cytoplasm. Membrane |
Application
Fig1: Western blot analysis of PDPK1 on human PDPK1 recombinant protein using anti-PDPK1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PDPK1 on HEK293 (1) and PDPK1-hIgGFc transfected HEK293 (2) cell lysate using anti-PDPK1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of PDPK1 on different cell lysate using anti-PDPK1 antibody at 1/1,000 dilution. Positive control： Line1: MCF-7 Line2: Hela Line3: K562 Line4: U937 Line5: A549 Line6: NIH/3T3 Line7: Jurkat Line8: PC-12 Line9: Cos7
Application
Fig4: ICC staining PDPK1 (green) and Actin filaments (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded bladder cancer tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of A549 cells with PDPK1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PDPK1 recombinant protein, PDPK1-hIgGFc transfected HEK293 cell lysate, MCF-7, Hela, K562, U937, A549, NIH/3T3, Jurkat, PC-12, Cos7, bladder cancer tissue. |
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| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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