| 存储条件 |
|---|
| Product Name | SHC1 [7C-7F] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ26504 antibody p66 antibody p66SHC antibody SH2 domain protein C1 antibody SHC (Src homology 2 domain containing) transforming protein 1 antibody SHC 1 antibody SHC A antibody SHC adaptor protein 1 antibody Shc antibody SHC transforming protein 1 antibody SHC transforming protein antibody SHC-transforming protein 1 antibody SHC-transforming protein 3 antibody SHC-transforming protein A antibody SHC1 antibody SHC1_HUMAN antibody SHCA antibody Src homology 2 domain-containing-transforming protein C1 antibody |
| Product description | Growth factor triggering of protein tyrosine kinase receptors induces signals that cascade to the nucleus activating mitogenic, as well as other, responses. Critical components of this process include adapter proteins such as Shc and IRS-1 that lack detectable catalytic activity. These are immediate substrates of receptor tyrosine kinase activity and serve to physically link activated receptors to downstream signaling components. Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate (4-7). Shc also participates in insulin signaling by linking the insulin receptor to Ras by forming complexes with the adapter protein GRB2 and Sos independently of IRS-1. A protein immunologically related to IRS-1, originally designated 4PS and now known as IRS-2, was shown to become highly tyrosine phosphorylated in response to IL-4 or IGF-1 in cells lacking IRS-1. An additional member of this family of signaling intermediates, Shb, is a SH2-containing protein with characteristic proline-rich domains. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | FLJ26504 antibody p66 antibody p66SHC antibody SH2 domain protein C1 antibody SHC (Src homology 2 domain containing) transforming protein 1 antibody SHC 1 antibody SHC A antibody SHC adaptor protein 1 antibody Shc antibody SHC transforming protein 1 antibody SHC transforming protein antibody SHC-transforming protein 1 antibody SHC-transforming protein 3 antibody SHC-transforming protein A antibody SHC1 antibody SHC1_HUMAN antibody SHCA antibody Src homology 2 domain-containing-transforming protein C1 antibody |
|---|---|
| Molecular Weight(MW) | 63 kDa |
| Cellular Localization | Cytoplasm, Mitochondrion matrix. |
Application
Fig1: Western blot analysis of SHC1 on?human SHC1 recombinant protein using anti-SHC1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of SHC1 on HEK293 (1) and SHC1-hIgGFc transfected HEK293 (2) cell lysate using anti-SHC1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of SHC1 on NIH/3T3?cell lysate using anti-SHC1 antibody at 1/1,000 dilution.
Application
Fig4: ICC staining SHC1 (green) and Actin filaments (red) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of NIH/3T3?cells with SHC1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human SHC1 recombinant protein, SHC1-hIgGFc transfected HEK293 cell lysate, NIH/3T3, A431. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
抱歉，暂无相关文献
